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AP Biology is a college-level course and exam offered to high school students through the College Board’s Advanced Placement (AP) program. It is designed to provide students with a rigorous foundation in biological principles, equivalent to an introductory college biology course for science majors.
The AP Biology 2025 exam tested students on foundational biological concepts, scientific inquiry, and data analysis.
Click on the following image to get the complete paper of AP Biology 2025 Free Response Questions. Scroll down the page for step-by-step solutions.
AP Biology 2025 Free Response Questions - Complete Paper (pdf)
2025 AP Biology Free Response Solutions for Questions 1 to 6
Most proteins that are secreted from a cell must be transported to the endoplasmic reticulum (ER) either during translation or after translation. …
Many insects rely on pheromones (chemical signals) that are released by the females to find mating partners. Scientists hypothesize that, in a certain type of moth, the behavior of male moths in response to pheromones is regulated by the extracellular signaling molecule 20E. …
Buffelgrass, an invasive grass species in southwestern desert ecosystems, is threatening the saguaro cactus, a keystone species in these ecosystems. Buffelgrass is drought-tolerant and can survive wildfires. However, the dry buffelgrass also acts as fuel for wildfires, causing the fires to be more severe. Older saguaro cacti can survive wildfires; however, many of the young cacti cannot. …
Twenty million years ago the Caribbean Sea and Pacific Ocean were connected, and water flowed freely between the two bodies of water. Many of the same marine species were found in both areas. Over millions of years, the land referred to as the Isthmus of Panama formed, eventually closing off the connection between the Caribbean Sea and Pacific Ocean and creating two separate bodies of water. The ecology of these two marine habitats was dramatically altered by this land formation. The warmer Caribbean water could no longer flow west, so the Pacific water cooled and became more nutrient-rich, while the Caribbean water became warmer. …
Figure 1 shows the reactions of the metabolic pathway used to synthesize amino acid B from amino acid A in cells.
The ald gene of fruit flies encodes the ALD protein, which is associated with both the centromeres of chromosomes and protein filaments produced during meiosis. In the absence of functional ALD proteins, gamete-producing cells enter anaphase I before homologous chromosomes are correctly aligned. As a result, the gametes produced do not contain the correct numbers of chromosomes
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