Biology Required Practical: pH & Enzyme Activity

GCSE Biology Required Practical - pH & Enzyme Activity

Investigate the effect of pH on the rate of reaction of amylase enzyme.

• Students should use a continuous sampling technique to determine the time taken to completely digest a starch solution at a range of pH values.
• Iodine reagent is to be used to test for starch every 30 seconds.
• Temperature must be controlled by use of a water bath or electric heater.

Enzymes - GCSE Science Required Practical
How to measure the rate of reaction between amylase and starch at different pH?

Required Practical: Effect of pH on Amylase
How to investigate the effect of pH on the enzyme amylase?

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Check out the sample question and solution on Sample Assessment Material (page 13), paper 1BI0/1BH and 1BIO/1BF.

Phenolphthalein is an indicator. It is pink in alkaline solutions and turns colourless as the pH decreases.
It can be used to measure the activity of the enzyme lipase on the breakdown of lipids.
Samples of milk containing phenolphthalein were incubated with lipase at different temperatures.
The time taken for the phenolphthalein to turn colourless was recorded and used to calculate the rate of enzyme activity.

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