Biology Required Practicals
Investigate the effect of pH on the rate of reaction of amylase enzyme.
Enzymes - GCSE Science Required Practical
How to measure the rate of reaction between amylase and starch at different pH?
Required Practical: Effect of pH on Amylase
How to investigate the effect of pH on the enzyme amylase?
Check out the sample question and solution on Sample Assessment Material (page 13), paper 1BI0/1BH and 1BIO/1BF.
Phenolphthalein is an indicator. It is pink in alkaline solutions and turns colourless as the pH decreases.
It can be used to measure the activity of the enzyme lipase on the breakdown of lipids.
Samples of milk containing phenolphthalein were incubated with lipase at different temperatures.
The time taken for the phenolphthalein to turn colourless was recorded and used to calculate the rate of enzyme activity.
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