Investigate how paper chromatography can be used to separate and tell the difference between coloured substances. Students should calculate Rf values.
Paper chromatography is the separation of mixtures of soluble substances by running a solvent (mobile phase) through the mixture on the paper (the paper contains the stationary phase), which causes the substances to move at different rates over the paper
In this practical students use paper chromatography to separate and identify a mixture of food colourings.
Paper Chromatography - GCSE Science Required Practical
How to separate substances using chromatography and measure Rf values.
00:00 Preparing experiment
06:33 Analysing results
07:57 Calculating Rf value
How to use paper chromatography to identify the colours in a sample of food colouring?
Check out the sample question and solution on Sample Assessment Material (page 56).
This example is taken from the sample assessment material, paper 1CH0/1H, Q1.
Mixtures of coloured substances can be separated by paper chromatography.
(a) Paper chromatography was used to separate a mixture of blue and red inks.
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