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Polymerase Chain Reaction

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Videos and experiments that are suitable for Biology. How the Polymerase Chain Reaction amplifies a specific DNA fragment from a complex mixture?

Polymerase Chain Reaction (PCR)
The polymerase chain reaction, or PCR, amplifies a specific DNA fragment from a complex mixture. This video explains the process.
Polymerase Chain Reaction (PCR)
Polymerase chain reaction (PCR) allows researchers to amplify DNA in a test tube. This process uses an enzyme derived from heat-resistant bacteria. The steps of PCR are driven by changes in temperature. Originally created for DNA Interactive TRANSCRIPT: Polymerase chain reaction (PCR) is a process where many copies of a specific piece of DNA can be made. This is known as amplification. Double-stranded DNA (red) unwinds and separates when the temperature is increased. As the temperature is decreased, small starter sequences called primers (glowing) can attach or anneal to the DNA. These primer sequences are usually only 20 to 25 nucleotides long, and are designed to match the start and end points of the DNA piece to be amplified. Once the primers have annealed, Taq polymerase (blue) copies the DNA starting from the primer. The temperature is increased; the strands separate; more primers anneal; the DNA is copied; and this cycle is repeated many times. In a typical PCR reaction there are 30 cycles, which can potentially create one billion copies starting from one molecule of DNA.

Polymerase Chain Reaction (PCR)
Polymerase chain reaction (PCR) enables researchers to produce millions of copies of a specific DNA sequence in approximately two hours. This automated process bypasses the need to use bacteria for amplifying DNA.
Polymerase chain reaction
Short video to describe the basics of polymerase chain reaction or PCR. This is just general information to give an idea of what PCR is.

Polymerase chain reaction (PCR)

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